Remote fiber-optic biosensors based on evanescent-excited fluoro-immunoassay: Concept and progress
- 1 July 1985
- journal article
- Published by Institute of Electrical and Electronics Engineers (IEEE) in IEEE Transactions on Electron Devices
- Vol. 32 (7) , 1175-1179
- https://doi.org/10.1109/t-ed.1985.22096
Abstract
A homogeneous silica optical fiber with its coating and cladding removed will interact with its surroundings via evanescent-wave modes at the interface. Antibody or antigen molecules can be covalently linked to the silica-fiber surface. The immobilized biomolecules will bind complementary antigen or antibody from a surrounding solution. If the bound antigen or antibody is fluorescent, a fluoro-immunoassay can be performed. The sensitivity of such a sensor is enhanced in a competitive immunoassay mode, where a fluorescently labeled antigen or antibody competes for the binding sites on the immobilized bio-molecules, thereby providing a competitive binding fluoro-immunoassay. Such sensors have the potential for remote unattended pseudocontinuous monitoring of biomolecules.Keywords
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