EVALUATION OF MINITEC AND API AS RAPID DIAGNOSTIC METHODS FOR ANAEROBIC-BACTERIA

  • 1 January 1984
    • journal article
    • research article
    • Vol. 19, 103-111
Abstract
The performance of the miniaturized biochemical differentiation systems API 20 Anaerobe System and Minitek was compared with pH reactions in prereduced anaerobically sterilized media for 58 anaerobic or capnoaerophilic bacterial strains from recent clinical specimens. The overall correlation of the Minitek reactions was 87% and of the API reactions 85%. Acid formation from carbohydrates was better detected by Minitek. This resulted in a correct diagnosis in 90% of cases after use of Minitek and 86% after use of API. If corrected for 3 strains of Clostridium perfringens for which double hemolysis prevented another diagnosis, 95% of the strains were correctly identified by Minitek compared to 91% with API. The errors were mostly due to lack of sensitivity of the reactions in the test kits.

This publication has 0 references indexed in Scilit: