Formation of Multilayered Vesicles from Water/Organic-Solvent (W/O) Emulsions: Theory and Practice

Abstract
Multilayered liposome (MLV) formation from water/organic-solvent (W/O) emulsions was studied. A fundamental liposome population parameter, the Eratio, was defined and used to estimate the bilayer number and water spacing in MLV liposomes. MLVs prepared from W/O emulsions have optimum drug entrapment at an emulsion-lipid/emulsion-water ratio of ∼0.33. Drug entrapment is typically 50 to 65% under these optimal conditions.