NITROGEN FIXATION BY PSEUDOMONAS-LIKE SOIL BACTERIA

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Abstract
Bacteria capable of growing on non-nitrogenous media were isolated from Idaho and Washington soils. These organisms were capable of fixing atmospheric N as demonstrated by semimicro-Kjeldahl determinations and isotopic tracer studies. Colonies of these organisms on non-nitrogenous agar are punctiform, hemispherical, transparent becoming translucent, smooth, entire, and cohesive with a hardened outer gum layer. A small amount of yellow to orange pigment is formed. The individual cells are slender, polar flagellated, nonsporulating, Gram-negative rods, 0.5 by 2-4 [mu]. Physiologically, the isolates are aerobic with no conspicuous fermentative properties. It is suggested that these organisms be placed in the family Pseudomonadaceae, Appendix I of Xanthomonas, and designated Pseudomonas azotocolligans, nov. sp.

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