STIMULATION OF HUMAN MONOCYTE MACROPHAGE-DERIVED GROWTH-FACTOR (MDGF) PRODUCTION BY PLASMA FIBRONECTIN

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  • 1 January 1983
    • journal article
    • research article
    • Vol. 111  (3) , 367-373
Abstract
Culture supernatants from human peripheral blood monocytes, isolated free of platelet contamination and cultured in the absence of serum, stimulate DNA synthesis and cell growth in Balb/c [mouse] 3T3 fibroblasts and bovine aortic smooth-muscle cells. Monocytes cultured in serum-free medium for 24 h with plasma fibronectin, added as either a surface-attached or soluble molecule, secrete significantly increased amounts of growth-promoting activity. Fibronectin also stimulates an increase in intracellular growth factor content and in protein synthesis by monocytes. Both the enhanced growth-promoting activity and protein synthesis are inhibited by cycloheximide. Thus, fibronectin-monocyte interactions may influence the production of growth-promoting activity by monocytes and contribute to fibroblast and smooth-muscle replication in wound healing, chronic inflammation and atherosclerosis.