skippy, a retrotransposon from the fungal plant pathogen Fusarium oxysporum
- 1 November 1995
- journal article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 249 (6) , 637-647
- https://doi.org/10.1007/bf00418033
Abstract
A retrotransposon from the fungal plant pathogen Fusarium oxysporum f. sp. lycopersici has been isolated and characterized. The element, designated skippy (skp) is 7846 by in length, flanked by identical long terminal repeats (LTR) of 429 by showing structural features characteristic of retroviral and retrotransposon LTRs. Target-site duplications of 5 bp were found. Two long overlapping open reading frames (ORF) were identified. The first ORF, 2562 by in length, shows homology to retroviral gag genes. The second ORF, 3888 bp in length, has homology to the protease, reverse transciptase. RNase H and integrase domains of retroelement pol genes in that order. Sequence comparisons and the order of the predicted proteins from skippy indicate that the element is closely related to the gypsy family of LTR-retrotransposons. The element is present in similar copy numbers in the two races investigated, although RFLP analysis showed differences in banding patterns. The number of LTR sequences present in the genome is higher than the number of copies of complete elements, indicating excision by homologous recombination between LTR sequences.Keywords
This publication has 30 references indexed in Scilit:
- Occurrence of a retrotransposon-like sequence among different formae speciales and races of Fusarium oxysporumMycological Research, 1994
- Tadl-1, an active LINE-like element of Neurospora crassaMolecular Genetics and Genomics, 1994
- Transposable elements in the fungal plant pathogenFusarium oxysporumGenetica, 1994
- Grasshopper,a Long Terminal Repeat (LTR) Retroelement in the Phytopathogenic FungusMagnaporthe griseaMolecular Plant-Microbe Interactions®, 1993
- Gene organization and transcription of TED, a lepidopteran retrotransposon integrated within the baculovirus genome.Molecular and Cellular Biology, 1990
- Host species-specific conservation of a family of repeated DNA sequences in the genome of a fungal plant pathogen.Proceedings of the National Academy of Sciences, 1989
- DNA damage activates transcription and transposition of yeast Ty retrotransposonsMolecular Genetics and Genomics, 1989
- Origins and Evolutionary Relationships of RetrovirusesThe Quarterly Review of Biology, 1989
- Amino acid sequence homology in gag region of reverse transcribing elements and the coat protein gene of cauliflower mosaic virusNucleic Acids Research, 1986
- Activation regions in a yeast transposon have homology to mating type control sequences and to mammalian enhancers.Proceedings of the National Academy of Sciences, 1985