Somatomedin C/insulin-like growth factor I: simplified purification procedure and biological activities of the purified growth factor
- 1 July 1986
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 110 (1) , 151-158
- https://doi.org/10.1677/joe.0.1100151
Abstract
Human somatomedin C has been purified from Cohn fraction IV paste by a simplified procedure using chromatofocusing, hydroxylapatite chromatography and reverse-phase high performance chromatography. The purified material has a specific activity by somatomedin C radioimmunoassay of 9160 units/mg (1 unit is defined as the amount of somatomedin present in 1 ml normal adult male human serum), representing a 650 000-fold purification, and possesses sulphation, mitogenic and insulin-like activities (specific activities of 3388 units/mg, 832 units insulin equivalents/mg and 1122 units/mg respectively). Somatomedin C is shown to be a potent stimulator of DNA synthesis (50% maximum stimulation at 150 fmol/ml) in isolated chondrocytes derived from costal cartilage, a major physiological target tissue. J. Endocr. (1986) 110, 151–158This publication has 10 references indexed in Scilit:
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