A Simple, Reliable, and Fast Protocol for Thraustochytrid DNA Extraction
- 1 March 2001
- journal article
- Published by Springer Nature in Marine Biotechnology
- Vol. 3 (2) , 100-102
- https://doi.org/10.1007/s101260000069
Abstract
DNA extraction of thraustochytrids, common marine unicellular organisms, is usually accomplished by either the cetyltrimethylammonium bromide (CTAB) or proteinase K protocols. A novel lysis buffer protocol for thraustochytrid total DNA extraction is described. The average isolated total DNA is 20 to 40 kb, and DNA samples are suitable for a variety of uses including 18S-ribosomal DNA polymerase chain reaction, restriction enzyme digestions, and amplified fragment length polymorphism analyses. The new protocol is also faster than the other protocols.Keywords
This publication has 0 references indexed in Scilit: