Antibody determination by ELISA in rats with retinal S antigen-induced uveoretinitis
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Current Eye Research
- Vol. 2 (1) , 7-12
- https://doi.org/10.3109/02713688208998375
Abstract
Enzyme-linked immunosorbent assay was applied to the determination of the serum IgG antibody contents in rats immunized with the organ-specific autoantigen (S antigen) of the retina. Optimal conditions (i.e. S antigen concentration, serum and conjugate dilutions, enzymatic reaction time) were determined. The assay required only a single serum dilution and was well reproducible. It was very sensitive, allowing the detection of low antibody contents in animals immunized with 1 μg of S antigen. The time course of the antibody response and its variations according to the dose and the species of the immunizing S antigen were considered.Keywords
This publication has 1 reference indexed in Scilit:
- S antigen-induced experimental autoimmune uveo-retinitis in ratsCurrent Eye Research, 1981