Simultaneous Determination of Creatinine and Uric Acid in Serum by High Performance Ion Exchange Chromatography with Direct Injection
- 1 July 1992
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 15 (9) , 1565-1575
- https://doi.org/10.1080/10826079208018309
Abstract
An anion-exchange chromatographic method for simultaneous determination of creatinine and uric acid in human serum is described. Serum samples were analyzed by direct injection high performance liquid chromatography which a column of quaternized resin cross-linked with ethylene glycol dimethacrylate (PCMS-Q). This allowed all proteins to elute at void volume. Thus, small analytes were readily separated from macromolecules. The method was found to be precise and accurate. There was a good correlation between the results of the new method and those of spectrophotometric methods for measuring creatinine and uric acid in human serum.Keywords
This publication has 10 references indexed in Scilit:
- Semipermeable-surface reversed-phase media for high-performance liquid chromatographyJournal of Chromatography A, 1991
- High-performance liquid chromatography packing materials for the analysis of small molecules in biological matrices by direct injectionJournal of Chromatography A, 1991
- Determination of creatinine in serum by high-performance liquid chromatography: A comparison of three ion-exchange methodsJournal of Chromatography B: Biomedical Sciences and Applications, 1990
- Direct plasma injection using internal surface reversed-phase high-performance liquid chromatography: feasibility study using propofol as a model compoundJournal of Chromatography B: Biomedical Sciences and Applications, 1988
- Simultaneous Determination of Ascorbic Acid and Uric Acids in Body Fluids by High-Performance Liquid Chromatography with Electrochemical DetectionJournal of Liquid Chromatography, 1986
- Simultaneous Determination of Ascorbic Acid, Isoascorbic Acid (Erythorbic Acid) and Uric Acid in Human Plasma by High-Performance Liquid Chromatography with Amperometric DetectionJournal of Liquid Chromatography, 1985
- Liquid chromatographic determination of creatinine in serum and urineAnalytical Chemistry, 1981
- HEMODIALYZER EVALUATION BY EXAMINATION OF SOLUTE MOLECULAR SPECTRAAsaio Journal, 1972
- Automated Determination of Uric Acid, with Use of a Uricase—Peroxidase SystemClinical Chemistry, 1971