Segregation distortion and linkage studies in microspore-derived double haploid lines of Hordeum vulgare L.

Abstract
A total of 62 doubled haploid (DH) lines was derived from a cross between tow lines of barley by anther culture. By two-dimensional electrophoresis of seedling proteins, the segregation of 28 loci in the population of DH lines was studied and a linkage map was constructed. The linkage map covered a large part of the length of the genome. A deviation to the 1∶1 segregation expected in the absence of selection was observed for at least one chromosome segment. This might be linked to a gene or group of genes selected because of their involvment in the process of haploid production.