HYDROXYLAPATITE MICROMETHOD FOR MEASURING ESTROGEN-RECEPTOR IN HUMAN BREAST-CANCER

  • 1 January 1978
    • journal article
    • research article
    • Vol. 38  (8) , 2216-2220
Abstract
The concentration of estrogen receptor (ER) in a human breast tumor is a critical variable predicting the response to endocrine therapy and the course of the disease. Since many tumor specimens are quite small, a reliable and simple ER assay requiring a minimum of tissue is desirable. A hydroxyapatite assay for ER that requires only a single, saturating concentration of [3H]estradiol, agrees with more complex multiple concentration assays and with the standard dextran-coated charcoal assay at normal protein concentrations, is more reliable than the latter at low protein concentrations and cen be adapted to an accurate and reliable ER microassay requiring less than 50 mg of tissue was described.

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