Effect of tumor necrosis factor on cell proliferation kinetics and sister chromatid exchange frequency in human lymphocytes
- 1 July 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 12 (7) , 1355-1357
- https://doi.org/10.1093/carcin/12.7.1355
Abstract
Recombinant human tumor necrosis factor α (TNF) was found to cause a significant increase in cell proliferation rates and sister chromatid exchange (SCE) frequency in phytohemagglutinin-stimulated human lymphocytes in vitro. The cells were treated for the entire cultivation time with 10, 50, 100, 500, 1000 and 5000 U/ml TNF. Cell proliferation rates, as measured by the replication index, increased significantly (P < 0.01) in a concentration-independent manner. The maximal extent of SCE induction (15.18 ± 0.57 versus 10.26 ± 0.45 SCEs/cell in control, P < 0.001) was observed with 50 U/ml TNF.Keywords
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