Effect of Trypsin on Agglutinability of Lipopolysaccharide Treated Erythrocytes

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Abstract
A study on hemagglutination of red blood cells from alligator, axolotl, snake, fish, sheep, and man modified by crude enterobacterial antigens and Escherichia coli lipopolvsaccharide (5 [mu]g/ml) revealed that hemagglutination of antigen-modified erythrocytes from the above cold-blooded animals does not take place in the presence of homologous antibodies, either at 37, 22 or 4 C. Under identical conditions hemagglutination is readily demonstrated with human and sheep red blood cells. Trypsin treatment of alligator and axolotl cells either before or after modification results in hemagglutination upon addition of homologous bacterial antibodies. It is concluded that trypsin removes an inhibitor interfering with the agglutination reaction per se and not with the attachment of the antigens to the red blood cells of alligator and axolotl.