The micropropagation of gooseberry (Ribes uva-crispaL.): II.In vitroproliferation andin vivoestablishment

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Abstract
Summary Proliferating shoot cultures of two gooseberry cvs, Invicta and Careless, were maintained in vitro for over 18 months before being rooted and re-established in vivo. The concentrations of KNO3 and NH4NO3 in the Murashige and Skoog medium were varied, and levels of 6.7 mM for both were found optimal for shoot proliferation when compared with eight other combinations. This is considerably lower than the 20.6 mM of KNO3 and NH4NO3 contained in the full MS medium. Invicta shoots proliferated more slowly than Careless shoots and their proliferation rates were more variable. Shoots of both gooseberry cultivars were rooted and established in vivo, though only a few Invicta plants survived this stage. A comparison of in vitro- and in vivo-derived Invicta plants showed that more shoots developed at the base of in vitro-derived bushes and that these bushes also had more thorns.

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