The expression of anti-poly(LGlu60, LPhe40) idiotypic determinants dictated by the gene products in the major histocompatibility complex.

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Abstract
Antibodies raised in SWR/J mice (H-2q, Igc) to the random copolymer poly(LGlu60, LPhe40) (GPhe) were purified by immunoadsorbent chromatography and used to immunize a New Zealand red rabbit. The rabbit anti-idiotypic antiserum thus produced strongly inhibited the binding of 125I-GPhe by anti-GPhe antisera produced only in mice of H-2q haplotype, and had no effect on the binding of GPhe by anti-GPhe antisera produced in mice of other haplotypes, namely, H-2k and H-2p. The anti-idiotypic antiserum also inhibited the binding of GPhe by anti-GPhe-methylated bovine serum albumin antisera produced only in mice of H-2q haplotype. No linkage to Ig allotype was observed. The anti-GPhe antisera produced in F1 mice of (H-2q .times. H-2d) and (H-2q .times. H-2b) haplotypes have antibodies that are inhibited by the anti-idiotypic antiserum demonstrating the dominant presence in these F1 mice of idiotypic determinants whose expression is dictated by H-2q. The anti-idiotypic antiserum also inhibited the binding of 125I-poly(LGlu56, LLys35, LPhe9) (GLPhe) and 125I-GPhe antisera produced only in mice of H-2q haplotype. These specificites were also confirmed by the inhibition of the plaque-forming cells. The antibodies produced in mice of H-2q haplotype against GPhe and GLPhe share common idiotypic determinants that are recognized by the anti-idiotypic antiserum. A possible explanation for the unique findings of the expression of anti-GPhe idiotypic determinants in mice of H-2q haplotype are dictated by the gene product of the MHC (major histocompatibility complex) is that the macrophages in mice of H-2q haplotype present unique determinants of GPhe polymer in the response process to GPhe.

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