Over‐expression and refolding of β‐subunit from the chloroplast ATP synthase
- 17 February 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 298 (1) , 69-73
- https://doi.org/10.1016/0014-5793(92)80024-b
Abstract
We established a bacterial system for high-level over-expression of the spinach chloroplast atpB gene which encodes the ATP Synthase β subunit. Upon induction, atpB was expressed as at least 50% to 70% of total cell protein. Although the over-expressed β polypeptide formed insoluble inclusion bodies, more than fifty percent of it was restored to a functional form by solubilizing the inclusion bodies with 4 M urea and slowly removing the urea by stepwise dialysis. The resulting β subunit exhibited specific and selective nucleotide binding properties identical to those of the native β subunit.Keywords
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