Differential synthesis of blocked and unblocked 5'-termini in reovirus mRNA: effect of pyrophosphate and pyrophosphatase.
- 1 October 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (10) , 3448-3452
- https://doi.org/10.1073/pnas.73.10.3448
Abstract
Viral mRNA synthesized in vitro by reovirus-associated enzymes contained 5''-terminal ppG, GpppG or m7GpppGm. Selective synthesis of 1 type of 5''-end was obtained by adjusting the composition of the transcription reaction mixture based on a series of reactions proposed for formation of blocked, methylated 5''-terminal sequences of mRNA (cap formation). The presence of PPi increased the proportion of ppG 5''-ends, while addition of pyrophosphatase [from yeast] yielded mRNA with 5''-terminal GpppG, which in the presence of a methyl donor was converted to m7GpppGm.This publication has 32 references indexed in Scilit:
- Characterization of ribosome-protected fragments from reovirus messenger RNA.Journal of Biological Chemistry, 1976
- Processing of RNAAnnual Review of Biochemistry, 1976
- A unique RNA species involved in initiation of vesicular stomatitis virus RNA transcription in vitroCell, 1976
- Translational discrimination of ‘capped’ and ‘non‐capped’ mRNAS: inhibition by a series of chemical analogs of m7 GpppXFEBS Letters, 1976
- Evidence for role of m7G5′-phosphate group in recognition of eukaryotic mRNA by initiation factor IF-M3Nature, 1976
- The process of formation of the 5′-terminal modified structure in messenger RNA of cytoplasmic polyhedrosis virusFEBS Letters, 1976
- The modified 5′-terinal sequences in messenger RNA of mouse myeloma cellsJournal of Molecular Biology, 1975
- Nucleotide sequence of a viral RNA fragment that binds to eukaryotic ribosomesNature, 1975
- Methylated simian virus 40-specific RNA from nuclei and cytoplasm of infected BSC-1 cells.Proceedings of the National Academy of Sciences, 1975
- Methylation-dependent translation of viral messenger RNAs in vitro.Proceedings of the National Academy of Sciences, 1975