Escherichia coli B lacks one of the two initiator tRNA species present in E. coli K-12
- 1 December 1992
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 174 (23) , 7827-7830
- https://doi.org/10.1128/jb.174.23.7827-7830.1992
Abstract
We show that the metY locus which specifies tRNA(2fMet) in Escherichia coli K-12 specifies tRNA(1fMet) in E. coli B. This conclusion is based on results of Southern blot analysis of E. coli B and K-12 DNAs and on polymerase chain reaction amplification, cloning, and sequencing of an approximately 200-bp region of DNA corresponding to the metY loci of E. coli B and E. coli K-12. We also show that the metY locus of E. coli B is transcriptionally active. E. coli strains transformed with the multicopy plasmid vector pUC19 carrying the metY locus of E. coli B overproduce tRNA(1fMet) in E. coli B and E. coli K-12 in contrast to strains transformed with pUC19 carrying the corresponding locus from E. coli K-12, which overproduce tRNA(2fMet).Keywords
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