GENERATION OF SUPPRESSOR CELLS BY AGGREGATED HUMAN GLOBULIN

  • 1 January 1981
    • journal article
    • research article
    • Vol. 43  (2) , 351-356
Abstract
The capacity of aggregated human globulin [AHG], a model for immune complexes [IC] to induce suppressor cells which modulate a predominantly T cell response, the autologous mononuclear cell proliferative response to concanavalin A [con A] was studied. A soluble AHG preparation depleted of both high MW (> 4 .times. 106 daltons) insoluble aggregates and monomeric IgG was used. The mean per cent suppression induced by mononuclear cells preincubated with AHG for 96 h was 39 .+-. 8%; monomeric IgG did not induce significant suppression (4 .+-. 6%). A close correlation between the magnitude of suppression induced by preincubation for 96 h with AHG and that induced by preincubation with conA. Kinetic analysis indicated that suppressor cells induction by AHG required 48 h of preincubation; suppressor cell induction by conA required 24 h. AHG-induced suppression was not associated with DNA synthesis as measured by 3H-thymidine uptake. IC can modulate immunoregulation.