A simple test for erythropoietin
- 1 October 1977
- journal article
- research article
- Published by Springer Nature in Zeitschrift für Die Gesamte Experimentelle Medizin
- Vol. 171 (3) , 289-295
- https://doi.org/10.1007/bf01851513
Abstract
In Gelfiltration on Sephadex G-100 crude protein prepared from the urine of anemic patients incubated with59Fe+++-ions was separated and a distinct fraction isolated having high erythropoietic activity and a high59Fe+++-absorption. Increased amount of erythropoietin resulted in a higher incorporation of59Fe+++-ions in this fraction. From this we propose to replace the time consuming and very expensive bioassay for erythropoietin in polycythemic mice by gelfiltration of the material under investigation preincubated with59Fe+++-ions to test the Fe+++ uptake in the forementioned fraction.This publication has 13 references indexed in Scilit:
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