Neutralizing Antiserum to Thrombopoietin

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Abstract
Potent thrombopoietin (TSF) materials were mixed with normal rabbit serum and serum from rabbits that were immunized with TSF-rich fractions of human plasma, human urine or kidney cell culture media. The supernatant fluids from these incubations were then assayed for TSF content by means of the immunothrombocythemic mouse assay. TSF, after incubation with normal rabbit serum, stimulated platelet production in mice, whereas pretreatment of the TSF with anti-TSF serum neutralized the ability of TSF to stimulate thrombocytopoiesis in mice. The hypothesis that TSF found in kidney cell culture medium is immunologically similar to TSF from human urine or plasma is supported.

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