AphanoDB: a genomic resource for Aphanomyces pathogens

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Abstract
Background: The Oomycete genusAphanomycescomprises devastating plant and animal pathogens. However, little is known about the molecular mechanisms underlying pathogenicity ofAphanomycesspecies. In this study, we report on the development of a public database called AphanoDB which is dedicated toAphanomycesgenomic data. As a first step, a large collection of Expressed Sequence Tags was obtained from the legume pathogenA. euteiches, which was then processed and collected into AphanoDB.Description: Two cDNA libraries ofA. euteicheswere created: one from mycelium growing on synthetic medium and one from mycelium grown in contact to root tissues of the model legumeMedicago truncatula. From these libraries, 18,684 expressed sequence tags were obtained and assembled into 7,977 unigenes which were compared to public databases for annotation. Queries on AphanoDB allow the users to retrieve information for each unigene including similarity to known protein sequences, protein domains and Gene Ontology classification. Statistical analysis of EST frequency from the two different growth conditions was also added to the database.Conclusion: AphanoDB is a public database with a user-friendly web interface. The sequence report pages are the main web interface which provides all annotation details for each unigene. These interactive sequence report pages are easily available through text, BLAST, Gene Ontology and expression profile search utilities. AphanoDB is available from URL:http://www.polebio.scsv.ups-tlse.fr/aphano/.