Comparison of oxidative metabolism in starved, fat-fed and carbohydrate-fed rats

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Abstract
Rats were starved for 48 hr. or fed for 1 week on a high-fat or a high-carbohydrate diet. The effects of these dietary alterations on the rate of production of 14CO2 from trace amounts of [U-14C]glucose, [1-14C]palmitate or [1-14C]acetate administered intravenously were studied. The oxidation of [14C]glucose was most rapid in the carbohydrate-fed condition and was decreased significantly and to the same extent after starvation and after feeding with fat. Under all dietary regimes studied the maximum rate of elimination of 14CO2 from [14C]palmitate occurred within a few minutes after injection, but considerably more was oxidized after starvation and feeding with fat than after feeding with carbohydrate. Alterations in diet had no effect on the oxidation and high recovery of administered [14 C] acetate as 14CO2. Graphical analysis showed the presence of several exponential components in the 14CO2-elimination curves. In all studies a marked similarity in oxidative pattern was noted between the starved and the fat-fed rat.