Observations on the Microfilaria of Onchocerca Volvulus with Silver Stains

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Abstract
Summary A silver stain is described which facilitates the demonstration of microfilaria in tissues. In addition, the stain reveals details not shown by routine procedures. Additional study would suggest that the microfilaria leave the Onchocerca nodule by the perivascular tissue spaces and not through the capillaries themselves. Evidence is present to suggest that invasion of capillaries is blocked by prompt reaction and thrombosis and that the microfilaria may be destroyed in the ensuing reaction.