Effect of Acute Inflammatory Lung Injury on the Expression of Monocyte Chemoattractant Protein-1 (MCP-1) in Rat Pulmonary Alveolar Macrophages
- 1 August 1992
- journal article
- Published by American Thoracic Society in American Journal of Respiratory Cell and Molecular Biology
- Vol. 7 (2) , 134-139
- https://doi.org/10.1165/ajrcmb/7.2.134
Abstract
Using a well-characterized rat model of immune complex-mediated acute inflammatory lung injury, we determined that there is a time-dependent elaboration of monocyte chemotactic activity in bronchoalveolar lavage fluid. Monocyte chemotactic activity is also significantly enhanced in culture supernatants from pulmonary alveolar macrophages (PAMs) from injured rat lungs. Northern hybridization analysis revealed markedly increased expression of rat monocyte chemoattractant protein 1 (MCP-1) mRNA in PAMs obtained from rats with immune complex-induced lung injury. The increased expression of MCP-1 mRNA and associated increase in monocyte chemotactic activity present in culture supernatants of PAMs from injured rat lungs suggest that PAMs may participate in the pathogenesis of acute inflammatory lung injury by the secretion of monocyte chemoattractants including MCP-1.Keywords
This publication has 15 references indexed in Scilit:
- Human monocyte chemoattractant protein‐1 (MCP‐1) Full‐length cDNA cloning, expression in mitogen‐stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JEPublished by Wiley ,2001
- Molecular cloning of rat monocyte chemoattractant protein-1 (MCP-1) and its expression in rat spleen cells and tumor cell linesBiochemical and Biophysical Research Communications, 1991
- Disparate gene expression of chemotactic cytokines by human mononuclear phagocytesBiochemical and Biophysical Research Communications, 1990
- Purification and amino acid analysis of two human glioma-derived monocyte chemoattractants.The Journal of Experimental Medicine, 1989
- Complete amino acid sequence of a human monocyte chemoattractant, a putative mediator of cellular immune reactions.Proceedings of the National Academy of Sciences, 1989
- The Effect of Acute Inflammatory Lung Injury on the Respiratory Burst and Protein Kinase C Activity of Rat Pulmonary Alveolar MacrophagesAmerican Review of Respiratory Disease, 1989
- Pulmonary Alveolar Macrophage Function during Acute Inflammatory Lung Injury1–3American Review of Respiratory Disease, 1987
- In vivo damage of rat lungs by oxygen metabolites.Journal of Clinical Investigation, 1981
- A 48-well micro chemotaxis assembly for rapid and accurate measurement of leukocyte migrationJournal of Immunological Methods, 1980
- Acute Immunologic Pulmonary AlveolitisJournal of Clinical Investigation, 1974