Plant regeneration from root callus protoplasts of sour cherry (Prunus cerasus L.)
- 1 September 1990
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 9 (5) , 268-271
- https://doi.org/10.1007/bf00232299
Abstract
Callus protoplasts of sour cherry clone CAB4D entered sustained division in Murashige and Skoog's (1962) medium with 1-naphthaleneacetic acid, 6-beneylaminopurine and zeatin. Further to callusing, organogenesis was induced from the protoplastderived callus, in a basal regeneration medium with these same growth regulators at 0.01 mg/l, 2,0 mg/l and 0.05 mg/l, respectively. The regeneration pathway, from such callus, could be altered by adding different organic compounds to this medium. Casein hydrolysate, added alone, promoted rhizogenesis, with shoot buds regenerated from the protoplast-derived roots, while in a basal regeneration medium with casein hydrolysate and a group B vitamin mixture direct caulogenesis occurred.Keywords
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