Comparative analysis of polymorphism and chromosomal location of tomato microsatellite markers isolated from different sources
- 1 February 2002
- journal article
- research article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 104 (2) , 229-235
- https://doi.org/10.1007/s00122-001-0775-2
Abstract
Previously isolated tomato (Lycopersicon esculentum) microsatellite markers were mainly clustered in the centromeric heterochromatin and not located in euchromatic regions. To achieve a more-uniform distribution of microsatellite markers for genome mapping purposes, a set of tomato microsatellite markers containing dinucleotide simple sequence repeats were developed by screening genomic libraries enriched for single-copy sequences, and screening the tomato EST database. The tomato microsatellites isolated in these ways were characterized by combinations of different types of repeated motifs and they were polymorphic in a set of L. esculentum varieties detecting up to four alleles. A total of 20 markers were placed on the genetic map of tomato. Interestingly, all markers isolated from genomic libraries enriched for single-copy sequences by PstI-pre-digestion mapped into the centromeric regions. The majority of markers derived from EST sequences contained predominantly AT microsatellites and were located in euchromatic regions.Keywords
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