A Human In Vitro Granuloma Model Using Heat Killed Candidaalbicans Cells Immobilized on Plastic Culture Wells

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Abstract
A new model for studying the initial events of granuloma formation in vitro is presented using heat killed Candida albicans immobilized on the surface of plastic culture wells. Human monocytes were induced to accumulate and to proliferate, forming multinucleated giant cells (MGC) and epitheloid cells within 4 days of culture. Tumour necrosis factor‐α (TNF‐α), interleukin (IL)‐1β and IL‐6 were detected in culture supernatants. These monokines, and additionally macrophage colony stimulating factor (M‐CSF), were also detected immunocytochemically. The granuloma formation was inhibited by Dexamethasone (Dex), Pentoxifylline (POF), or interferon‐γ (IFN‐γ) in a dose‐dependent manner. Antibodies to M‐CSF reduced the granuloma formation to a great extent with a striking reduction of monocyte proliferation. Using antibodies to TNF‐α the authors found a complete inhibition of the granuloma including MGC formation and monocyte proliferation.

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