Liver tumours after single dose dimethylnitrosamine, low and high protein diet, and phenobarbitone

Abstract
A single dose of 15 mg/kg dimethylnitrosamine given to rats at the time of switch from low to high protein diet is an effective initiator of hepatic tumour formation. With phenobarbitone as promoter, a high yield of liver tumours is produced. The switch from low to high protein diet may be a way of enhancing tumour initiation hi many tissues.