MODULATION OF NATURAL CYTOTOXICITY BY ALLOANTIBODIES .1. ALLOANTISERA ENHANCEMENT OF CYTOTOXICITY OF MOUSE SPLEEN-CELLS TOWARD A HUMAN MYELOID CELL-LINE

  • 1 January 1979
    • journal article
    • research article
    • Vol. 123  (2) , 846-851
Abstract
Natural killer activity of spleen cells obtained from different strains of mice against the human myeloid leukemia cell line K562 and 2 mouse cell lines P815 and L1210 was measured by using the 4 h Cr release assay. The level of cytotoxic activity of spleen cells against the K562 target was usually less than 4% lysis. Treatment of the spleen cells with a specific anti-H-2 antiserum resulted in a dose-dependent augmentation of the degree of lysis of K562 cells. The augmentation of cytotoxic activity could be obtained by pre-treatment of the spleen cells antisera or by directly adding the antisera to the incubation medium. Anti-thy-1 and anti-immunoglobulin antisera had no enhancing effect under similar conditions. The specific alloantisera-treated spleen cells did not show any increase in cytotoxicity against P815 [mouse mastocytoma] and L1210 [mouse lymphoma] target cells. Spleen cells responsible for the alloantiserum-mediated augmentation of cytotoxicity against K562 [human myeloid leukemia] cells appear different from T [thymus-derived] or B [bone marrow-derived] cells as indicated by their resistance to anti-thy-1 and complement treatment and lack of adherence to nylon wool columns.