The F and 19-hydroxy F prostaglandins and their 8β-isomers in human seminal plasma: Data on chromatography and mass spectrometry

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Abstract
Human seminal plasma contains eight different prostaglandins belonging to the F series, i.e. PGF1α, PGF2α, 19-hydroxy-PGF1α, 19-hydroxy PGF2α, as well as the corresponding 8β-isomers. A systematic description of the mass spectrometric fragmentation of the 8β-isomers has not been previously reported. In the present study the prostaglandins were separated from human seminal plasma and analysed as methylester, trimethylsilylethers with gas chromatography mass spectrometry. All the eight F and 19-hydroxy F prostaglandins fragmented in the same manner, but the relative abundances of the formed ions varied in a typical way. Thus, the mass spectra of 8β-PGF1α and 8β-19-hydroxy PGF1α differed clearly from those of their 8α-isomers, which could be used for identification and should be considered in selected ion monitoring. The ratio between the seminal plasma levels of 8β- and 8α-isomers of 19-hydroxy F1α was much higher than the corresponding ratio for the PGF1α.