Isolation of a Pregnenolone Metabolite, Δ5-Pregnene-3α,16α,20α-triol, from the Urine of Normal Men

Abstract
A 3H-labeled 3α-hydroxy-Δ5 steroid, Δ5-pregnene-3α,16α,20α-triol, was isolated from the pooled glucuronidase-hydrolyzed extracts and 3H-labeled Δ5-pregnene-3β,16α,20αtriol from the pooled solvolyzed extracts of urine collected for a total of 24 days from 6 healthy young men, aged 22–30, 3 of whom had received a tracer dose of pregnenolone-7α-3H (3β-hydroxy-Δ5-pregnen-20-one) intravenously.

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