Synergistic action of cysteamine and BrdU-substituted DNA in the induction of sister chromatid exchanges
- 1 December 1980
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 81 (3) , 461-471
- https://doi.org/10.1007/bf00368156
Abstract
The effect of different BrdU-concentrations on the cysteamine-induced SCE-rate was investigated in V-79 Chinese hamster cell monolayer cultures. Both cysteamine and its auto-oxidation product cystamine act synergistically with BrdU in the induction of SCEs. A given concentration of these substances produces a low SCE-frequency at low BrdU-concentrations — but the incidence of SCEs is significantly increased at increased BrdU-concentrations. — Using one-cycle substitution experiments and the determination of the relative level of substitution by means of 3HBrdU-incorporation, this synergism was shown to depend on the BrdU incorporated in the DNA and on the extent to which this incorporation takes place.Keywords
This publication has 37 references indexed in Scilit:
- Induction of sister chromatid exchanges by BUdR is largely independent of the BUdR content of DNANature, 1980
- Factors influencing the frequency of mitomycin C-induced sister-chromatid exchanges in 5-bromodeoxyuridine-substituted human lymphocytes in cultureMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1978
- Sister-chromatid exchange induced by X-ray of human lymphocytes and the effect of l-cysteineMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1978
- DNA replication patterns of human chromosomes from fibroblasts and amniotic fluid cells revealed by a Giemsa staining techniqueCytogenetic and Genome Research, 1975
- Spontaneous sister chromatid exchanges detected by a BUdR-labelling methodNature, 1974
- Circular dichroism and thermal denaturation studies of chromatin and DNA from BrdU-treated mouse fibroblastsBiochemical and Biophysical Research Communications, 1974
- Sister chromatid exchanges induced by light flashes to 5-bromodeoxyuridine- and 5-iododeoxyuridine substituted Chinese hamster chromosomesExperimental Cell Research, 1974
- Die wirkung von thioguanin, hydroxylamin unde 5-bromdesoxyuridin auf menschliche chromosomen in vitroMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1967
- Interaction of Cystamine and Cystamine Derivatives with Nucleic Acids and NucleoproteinsInternational Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1965
- Action of sulfhydryl compounds on human leukocyte mitosis in vitroExperimental Cell Research, 1964