MLR blast cells generated in mutant‐standard strain combinations bind H‐2K and H‐2D antigens

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Abstract
Strains CBA (M523) ( = M523) and CBA differ by a mutation which has been mapped genetically into the K region of the H‐2 complex. Similarly, strains B 10.D2 (M504) ( = MS04) and B10.D2 differ in a mutation which occurred in the D region. The data presented in this study show that mixed lymphocyte culture in M523 anti‐ CBA and M504 anti‐B 10.D2 strain combinations leads to the release of membrane fragments from the stimulating cells and binding of these fragments by blast cells. The fragments always carry H‐2K (in the M523 anti‐CBA combination) or H‐2D (in the M504 anti‐B 10.D2 combination) antigens present in the stimulating and absent in the responding cells (antigens H‐2.60 and H‐2.40, respectively). Although Ia antigens may occasionally be present on the CBA membrane fragments, these antigens do not participate in the M523 anti‐CBA MLR stimulation. The data thus demonstrate that serologically detectable H‐2K and H‐2D antigens can induce a MLR and that a mutation can change properties of H‐2K or H‐2D molecules so that the alteration is detectable by both serological means and lymphocyte activation assays.