Cycloheximide Resistance in Carrot Culture: A Differentiated Function

Publisher Website
Google Scholar
Abstract
Cultured carrot cells grow as unorganized callus tissue in medium containing auxin. Upon removal of the auxin from the medium, they grow in an organized manner and differentiate into embryos. In the normal cell line, W001C, the callus growth can be inhibited by cycloheximide but the embyronic growth cannot. A variant cell line, WCH105, whose callus growth is resistant to cycloheximide, was isolated. The mechanism of cycloheximide resistance in embryos of both lines and in WCH105 callus was cycloheximide inactivation. In addition to auxin, bromodeoxyuridine can promote callus growth in carrot culture. Callus cultures maintained by bromodeoxyuridine behave the same as do those maintained by auxin. WCH105 callus is resistant; W001C callus is sensitive to cycloheximide inhibition. Except for the onset of embryogenesis, cycloheximide inactivation is expressed throughout the embyro developmental stages up to the plantlets. Cycloheximide inactivation is a function expressed in the differentiated, but not in the undifferentiated, tissues.

This publication has 3 references indexed in Scilit: