Immunoglobulin heavy chain toxicity in plasma cells is neutralized by fusion to pre-B cells.
- 1 November 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (22) , 7185-7188
- https://doi.org/10.1073/pnas.81.22.7185
Abstract
A plasma cell hybridoma frequently loses its IgH chain spontaneously but rarely is production of its L chain lost. Upon fusion to a pre-B-cell hybridoma that produces no Ig chain, the L chain is frequently lost. In cells without the L chain the H chain, which is derived from the plasma cell, is not chemically modified. Results indicate that, in pre-B cells, but not in plasma cells, there must be a mechanism that neutralizes the toxic effect of free H chain.This publication has 16 references indexed in Scilit:
- Immunoglobulin chain loss in hybridoma lines.Proceedings of the National Academy of Sciences, 1980
- Evidence that murine pre-B cells synthesise μ heavy chains but no light chainsNature, 1979
- Control of immunoglobulin secretion in the murine plasmacytoma line MOPC 315.The Journal of Experimental Medicine, 1978
- Murine heavy chain diseaseEuropean Journal of Immunology, 1978
- Early production of intracellular IgM by B-lymphocyte precursors in mouseNature, 1976
- B lymphocyte differentiation induced by lipopolysaccharide. I. Generation of cells synthesizing four major immunoglobulin classes.1975
- High resolution two-dimensional electrophoresis of proteins.Journal of Biological Chemistry, 1975
- Heavy Chain-Producing Variants of a Mouse Myeloma Cell LineThe Journal of Immunology, 1975
- Intracellular immunoglobulin chain synthesis in non-secreting variants of a mouse myeloma: Detection of inactive light-chain messenger RNAJournal of Molecular Biology, 1974
- Rate of Somatic Mutation in Immunoglobulin Production by Mouse Myeloma CellsProceedings of the National Academy of Sciences, 1971