False Spurs in Quantitative Immunoelectrophoresis

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Abstract
Antigens [Ag] [human and ape [Cercopithecus aethiops] .alpha.-2 macroglobulins] which show reactions of identity in double-diffusion tests in gel may cause spur formation in crossed immunoelectrophoretic techniques. To differentiate these misleading spurs from spurs of double-diffusion tests which show the true immunochemical relationship between antigens, the former type is denoted ''false spurs''. False spurs are often formed in tandem-crossed immunoelectrophoresis and fused rocket immunoelectrophoresis when Ag with different electrophoretic mobility are compared. On the basis of model experiments, criteria are set up for accepting a spur as a true spur, and procedures are described for avoiding formation of false spurs in quantitative immunoelectrophoresis.

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