The ligand recognized by ELAM‐1 on HL60 cells is not carried by N‐linked oligosaccharides

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Abstract
The sialyl Lewis‐x determinant is a ligand for ELAM‐1, a major adhesion molecule for HL60 cells and neutrophils. ELAM‐1 expression can selectively be induced on human umbilical vein endothelial cells (HUVEC) by tumor necrosis factor, interleukin 1 and lipopolysaccharide. The determinant sialyl Lewis‐x is found on both glycolipids as well as on glycoproteins. Using specific inhibitors of the biosynthesis of N‐linked glycosylated glycoproteins, we investigated whether N‐linked glycans or their modifications are involved in ELAM‐1‐dependent adhesion of HL60 cells to activated HUVEC. The inhibitors of glycoprotein processing N‐methyl‐deoxynojirimycin, 1‐deoxymannojirimycin and swainsonine did not affect ELAM‐1‐dependent adhesion. Complex‐type N‐linked glycans are not required for ELAM‐1‐mediated adhesion, and therefore the ligand for ELAM‐1 is most likely a glycolipid, or a glycoprotein carrying O‐linked oligosaccharides.