EVIDENCE FOR THE PRESENCE OF AN ENDOGENOUS INHIBITOR OF PROLYL HYDROXYLASE

  • 1 January 1980
    • journal article
    • research article
    • Vol. 29  (2) , 329-338
Abstract
Prolyl hydroxylase [EC 1.14.11.2] [thought to be involved in the fibrogenic process in alcoholic liver disease] was inhibited by an ultrafiltrate (> 30,000 MW) fraction isolated from skin and blood of neonatal and adult rabbits. This fraction also inhibited 2 other .alpha.-ketoglutarate requiring mixed function oxidases, lysyl hydroxylase [EC 1.14.11.4] and .alpha.-butyrobetaine hydroxylase [EC 1.14.11.1] but not the amine oxidase, lysyl oxidase. Purification of the skin ultrafiltrate on Sephadex G-25 demonstrated a peak of prolyl hydroxylase inhibitory activity which chromatographed at a MW corresponding to approximately 3000. Chromatography of a blood ultrafiltrate separated a similar peak of material which was inhibitory for prolyl hydroxylase.