A Novel Method for Isolating Chlorate-resistant Mutants of Escherichia coli K12 by Anaerobic Selection on a Lactate plus Fumarate Medium

1 March 1976

journal article
research article
Published by Microbiology Society in Journal of General Microbiology

Vol. 93 (1) , 173-176
https://doi.org/10.1099/00221287-93-1-173

Abstract

Under anaerobic conditions E. coli is unable to grow in minimal medium with non-fermentable substrates unless an electron acceptor such as nitrate or fumarate is provided. Attempts to isolate E. coli mutants (frd) deficient in fumarate reductase on a lactate plus fumarate medium under anaerobic conditions led to a new method for the direct selection for chlorate-resistant mutants (chl).