A comparative, vapor‐phase (G.L.C.‐M.S.) study of various derivatives of prostaglandins A and B

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Abstract
The isomeric prostaglandins, A and B, can be readily distinguished by differences in the mass spectra of their derivatives. The mass spectra of the PGA1‐ or PGA2‐methyl ester (ME)‐trimethyl silyl (TMS) ether derivatives have a prominent ion at [M − 71]+ or [M − C5H11]+ while those of the PGB1‐ or PGB2‐ME‐TMS derivatives have a predominant ion at [M − 99]+ or [M − C6H11O]+ in addition to that at [M − 71]+. Ions of similar origin characterize the spectra of the PGA1‐ or PGA2‐TMS ether‐TMS ester and PGB1‐ or PGB1‐TMS‐TMS derivatives, respectively. The fragmentation of other derivatives of PGA1, PGA2, PGB1 and PGB2 such as the ME‐t‐Bu‐DMS (t‐butyl‐dimethylsilyl ether); ME‐MO (methoxime)‐TMS; ME‐MO‐Ac (acetate), and ME‐Ac are also described comparatively. The composition of important ions was confirmed by deuterium labeling and/or high resolution mass spectroscopy, where appropriate. The potential advantages and limitations of the derivatives for quantitative analysis of prostaglandins by the specialized technique of multiple ion detection (MID) are described.