DISSIMILATION OF C 14 -LABELED GLUCOSE BY SERRATIA MARCESCENS

Publisher Website
Google Scholar
Abstract
The distribution of radioactivity, after dissimilation of C14-labeled glucose by S. marcescens in the presence and absence of sodium arsenite, was investigated. Sixty per cent of 1-C of glucose was converted to CO2 by an arsenite-resistant pathway, while 24% of 1-C was localized within the cell by an arsenite-sensitive pathway. In the absence of arsenite, 27% of 6-C was found in the CO2 and 54.5% was recovered in the cells. Arsenite considerably inhibited these reactions. The 2-C of glucose was converted to CO2 and cellular material by arsenite-sensitive and -insensitive pathways. In the absence of arsenite, pyruvic or 2-keto-gluconic acids were not found. In the presence of the inhibitor, 1.7 [mu]moles pyruvic and 0.6 umoles 2-ketogluconic acids were obtained from 2.5 [mu]moles glucose.