COMPARISON OF DIRECT ANTIGLOBULIN ROSETTING REACTION WITH DIRECT IMMUNOFLUORESCENCE IN DETECTION OF SURFACE-MEMBRANE IMMUNOGLOBULIN ON HUMAN PERIPHERAL-BLOOD LYMPHOCYTES
- 1 January 1978
- journal article
- research article
- Vol. 34 (3) , 533-538
Abstract
Comparisons were made between the direct antiglobulin rosetting reaction (DARR) and direct immunofluorescence (DIF) in the detection of surface membrane immunoglobulin [Ig] of human peripheral lymphocytes. The DARR was more sensitive and the results with this testing procedure (as opposed to those with the DIF) were not influenced by various treatments of the lymphocytes before testing, such as incubation in AB serum at 4.degree. C, incubation in serum-free medium at 37.degree. C or short exposure to acetate buffer at pH 4.0. The DARR (as opposed to the DIF) gave essentially the same results whether the red cell-linked antiglobulin was IgG or the F(ab'')2 preparation. With mixed rosetting for T [thymus-derived] and SmIg+ [surface membrane Ig containing] lymphocytes, there was only 1% or less null cells and only 5% or less lymphocytes rosetted with both marker red cells.This publication has 16 references indexed in Scilit:
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