An Alcohol-Acid-Fast Substance in Eggs of Schistosoma Mansoni

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Abstract
Summary A specific alcohol-acid-fast substance in eggs of Schistosoma mansoni is described, which is regularly demonstrable in tissues and in the stools. Specific staining improves detection of eggs and of rests of eggs in tissue sections. A modification of the Ziehl-Neelsen stain for stool smears is described. This modification is shown to yield a higher percentage of positivity than direct examination, and is recommended for routine use. Two varieties of acid-fast material are shown to exist in the egg, one of them in the capsule (Substance C), and the other in the miracidium (Substance M). The first of these stains constantly; the latter only when the capsule is intact. Substance C is shown to be resistant to drastic chemical treatments and to persist in the old lesions within the tissues even after the egg has become fragmented.

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