Assay of Streptomycin by the Paper-Disc Plate Method

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Abstract
A method is described for the quantitative determination of streptomycin by the filter paper disc, agar diffusion technique, using Bacillus subtilis as the test organism. Factors influencing the range sensitivity and precision of the method have been investigated. The composition and pH of the assay medium must be carefully controlled, samples should be buffered in the range of 7.6 to 8, a constant volume of sample must be applied to the disc, the enhancing effect of salts in the sample are minimized in the presence of 0.1 [image] phosphate buffer, a variety of organic reagents had little or no effect on the assay. The accuracy of the method is presented graphically, the standard error for a given sample is dependent on the number of replicate samples assayed. The assay procedure given has proven satisfactory for surface and submerged-culture beers and for prepns. obtained in isolation and purification processes. [long dash]R. L. Libby.

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