Two unusual budding bacteria isolated from a swimming pool

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Abstract
Two unusual strains of budding bacteria were isolated on a Millipore Pseudomonas Count Water Tester during routine monitoring of P. aeruginosa counts in a swimming pool. The 1st isolate was identified as Blastobacter sp. It was a yellow-pigmented, gram-negative rod-shaped organism with a polar holdfast by which it attached to solid surfaces or other cells to form rosettes. The cells reproduced by asymmetric division or budding at the free pole of the cell, producing motile daughter cells with a single polar flagellum. The 2nd isolate, which has not yet been identified, was a red-pigmented, gram-negative rod-shaped organism which produced .gtoreq. 1 buds at each pole of the cell. Cell division appears to occur by binary fission and by budding. Both organisms were strict aerobes, catalase and oxidase positive and did not produce acid from glucose in Hugh and Leifson medium.

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