Analysis of the peptide carrier in the scutellum of barley embryos by photoaffinity labelling
- 1 December 1991
- journal article
- Published by Springer Nature in Planta
- Vol. 186 (1)
- https://doi.org/10.1007/bf00201496
Abstract
The preparation of a phenylalanine analogue containing an azido group and its incorporation into dipeptides is described. Peptides modified in this way are taken up into barley (Hordeum vulgare L.) scutella via the previously characterized peptide-transport system. Photoactivation of modified peptides in the presence of isolated scutella resulted in irreversible inhibition of peptide uptake in a concentration-dependent manner. Transport of other solutes which share a common mechanism of energy coupling, but which are transported via distinct carriers, was not inhibited after photo-derivatization of scutella with the modified peptides. Derivatization of isolated scutellar tissue with a 14C-labelled peptide analogue, resulted in incorporation of label into two proteins of Mr = 54000 and 41000. Scutellar tissue from early-germinating seeds, which do not show active peptide uptake, did not incorporate label into these polypeptides. It is concluded that these proteins are components of the barley peptide-transport system.Keywords
This publication has 23 references indexed in Scilit:
- Intestinal absorption of dipeptides and β-lactam antibiotics. II. Purification of the binding protein for dipeptides and β-lactam antibiotics from rabbit small intestinal brush border membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1990
- Intestinal uptake of dipeptides and β-lactam antibiotics. I. The intestinal uptake system for dipeptides and β-lactam antibiotics is not part of a brush border membrane peptidaseBiochimica et Biophysica Acta (BBA) - Biomembranes, 1990
- Identification of an Mr 75 000 component of the H+/d-glucose cotransporter from Zea mays with monoclonal antibodies directed against the mammalian Na+/d-glucose cotransporterBiochimica et Biophysica Acta (BBA) - Biomembranes, 1989
- Amino Acid and Peptide Uptake in the Scutella of Germinating Grains of Barley, Wheat, Rice, and MaizePlant Physiology, 1989
- Identification of Membrane Protein Associated with Sucrose Transport Into Cells of Developing Soybean CotyledonsPlant Physiology, 1988
- Identification of identical binding polypeptides for cephalosporins and dipeptides in intestinal brush-border membrane vesicles by photoaffinity labelingBiochimica et Biophysica Acta (BBA) - Biomembranes, 1987
- Solubilization of Plant Membrane Proteins for Analysis by Two-Dimensional Gel ElectrophoresisPlant Physiology, 1986
- The Peptide Pools of Germinating Barley Grains: Relation to Hydrolysis and Transport of Storage ProteinsPlant Physiology, 1981
- Photoaffinity inhibition of dipeptide transport in Escherichia coliBiochemistry, 1978
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970