GSK-3β-regulated interaction of BICD with dynein is involved in microtubule anchorage at centrosome

Open Access
Publisher Website
Google Scholar
Abstract
Microtubule arrays direct intracellular organization and define cellular polarity. Here, we show a novel function of glycogen synthase kinase‐3β (GSK‐3β) in the organization of microtubule arrays through the interaction with Bicaudal‐D (BICD). BICD is known to form a complex with dynein–dynactin and to function in the intracellular vesicle trafficking. Our data revealed that GSK‐3β is required for the binding of BICD to dynein but not to dynactin. Knockdown of GSK‐3β or BICD reduced centrosomally focused microtubules and induced the mislocalization of centrosomal proteins. The unfocused microtubules in GSK‐3β knockdown cells were rescued by the expression of the dynein intermediate chain‐BICD fusion protein. Microtubule regrowth assays showed that GSK‐3β and BICD are required for the anchoring of microtubules to the centrosome. These results imply that GSK‐3β may function in transporting centrosomal proteins to the centrosome by stabilizing the BICD1 and dynein complex, resulting in the regulation of a focused microtubule organization.

This publication has 68 references indexed in Scilit: