Effect of pH on the growth cycle of HeLa cells in batch suspension culture without oxygen control
- 1 July 1971
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 13 (4) , 471-492
- https://doi.org/10.1002/bit.260130403
Abstract
HeLa cells have been cultured at different controlled pH levels in batch suspension culture with open gas phase. Continuous monitoring of the dissolved oxygen tension throughout the culture cycle reveals a characteristic pattern of variation which correlates with various aspects of growth and carbohydrate metabolism. The effect of pH, size, and physiological state of the inoculum on this pattern have been determined, and the results are discussed in terms of a theoretical respiratory rate equation.Keywords
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